Transgender: A Reference Handbook is a go-to resource about the transgender experience. The book takes contemporary as well as historic aspects into consideration. It looks at ancient indigenous cultures that honored third, fourth, and fifth gender identities as well as more contemporary ideas of what "transgender" means. Notably, it focuses not only on Western medical ideas of gender affirmation but on cultural diversity surrounding the topic.

This book will primarily serve as a reference guide and jumping off point for further research for those seeking information about what it means to be transgender. While a reference book, it contains original work that may be cited in addition to the encyclopedia itself. In particular, the perspectives section of the book includes writings from some of the world's foremost trans writers, activists, artists, and historians.